Autophagy Is Required for Hepatic Differentiation of Hepatic Progenitor Cells via Wnt Signaling Pathway

Figure 4

Autophagy regulated Wnt signaling pathway during hepatic differentiation of HPCs. (a) RT-PCR analyzing the mRNA expression of Wnt1, Wnt3a, Wnt4, Wnt5a, and Wnt9a in WB-F344 cells treated with differentiation medium (HGF) for 2 days and coculture DMSO or rapamycin (200 nM; Rapa) for 24 h. (b) RT-PCR analyzing the mRNA expression of β-catenin, Sox9, and Axin2 in WB-F344 cells treated with differentiation medium (HGF) for 2 days and coculture DMSO or rapamycin (200 nM; Rapa) for 24 h. (c) Western blot showing the level of Wnt5a, P-CaMKIIα, and CaMKIIα in WB-F344 cells treated with differentiation medium (HGF) for 2 days and coculture DMSO or rapamycin (200 nM; Rapa) for 24 h. (d) Western blot showing the level of Wnt3a, β-catenin, and Sox9 in WB-F344 cells treated with differentiation medium (HGF) for 2 days and coculture DMSO or rapamycin (200 nM; Rapa) for 24 h. (e) RT-PCR analyzing the mRNA expression of Wnt1, Wnt3a, Wnt4, Wnt5a, and Wnt9a in WB-F344 cells treated with differentiation medium (HGF) for 2 days and coculture DMSO or starvation (serum-free medium; Starve) for 24 h. (f) RT-PCR analyzing the mRNA expression of β-catenin, Sox9, and Axin2 in WB-F344 cells treated with differentiation medium (HGF) for 2 days and coculture DMSO or starvation (serum-free medium; Starve) for 24 h. (g) Western blot showing the level of Wnt5a, P-CaMKIIα, and CaMKIIα in WB-F344 cells treated with differentiation medium (HGF) for 2 days and coculture DMSO or starvation (serum-free medium; Starve) for 24 h. (h) Western blot showing the level of Wnt3a, β-catenin, and Sox9 in WB-F344 cells treated with differentiation medium (HGF) for 2 days and coculture DMSO or starvation (serum-free medium; Starve) for 24 h. Data represent ; ; . .

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