Transfer of Th17 from Adult Spontaneous Hypertensive Rats Accelerates Development of Hypertension in Juvenile Spontaneous Hypertensive Rats

Figure 2

T cell profiles of the peripheral blood mononuclear cells (PBMCs) and spleen in WKY and SHR. Quantification of flow cytometric analysis revealed the proportion of CD3⁺CD4⁺ (Th) cells of the (a) PBMCs and (b) spleen in juvenile and adult rats. The proportion of CD3⁺CD4⁺ (Th) cells of PBMCs was higher in WKY than in SHR. The proportion of CD3⁺CD4⁺ (Th) cells of the spleen revealed comparable in 5 weeks and 15 weeks, but increased at 15 weeks compared with 5 weeks (, vs. WKY; ^## vs. corresponding juvenile rats by two-way ANOVA followed by the post hoc Duncan test). Quantification of flow cytometric analysis revealed the proportion of CD4⁺IL-17A⁺ (Th17) and CD4⁺FoxP3⁺ (Treg) cells among CD3⁺CD4⁺ (Th) cells of the (c) PBMCs and (d) spleen in adult WKY and SHR by the gating strategy of flow cytometry. There was a higher proportion of CD4⁺IL-17A⁺ (Th17) cells of PBMCs in SHR than in WKY, and there was a lower proportion of CD4⁺FoxP3⁺ (Treg) cells in SHR than in WKY. Data are the of 6 independent experiments ( vs. WKY by Student’s -test).

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