TREM1 Blockade Ameliorates Lipopolysaccharide-Induced Acute Intestinal Dysfunction through Inhibiting Intestinal Apoptosis and Inflammation Response

Figure 1

TREM1 inhibitor LP17 attenuates the injury of the small intestine tissues in rats with LPS-induced acute intestinal dysfunction. Rats were randomly assigned into three groups: normal (), model (), and LP17 (). The rats in the model group and the LP17 group received intraperitoneal injection of 4.5 mg/kg LPS. The rats in the LP17 group received LP17 administration (3.5 mg/kg body weight) through the vena caudalis at the time of LPS injection, while the rats in the normal and model groups were injected with equal volume of saline. (a) Representative images of histopathological analysis of the small intestine tissues by H&E staining. Scale  μm. (b) The Chiu scores (), (c) length of villi (), and (d) mucosal thickness () of the small intestine were assessed in the indicated groups. (e, f) Representative images and quantitative results of immunohistochemistry for TREM1 expression () in the small intestine tissues of the indicated groups. Scale  μm. (g, h) Representative images and quantitative results of western blot showing the expression levels of TREM1 and the internal control gene β-actin in the rat small intestine tissues (). One-way analysis of variance (ANOVA) was used for multiple comparisons, followed by Tukey’s test. .

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