TREM1 Blockade Ameliorates Lipopolysaccharide-Induced Acute Intestinal Dysfunction through Inhibiting Intestinal Apoptosis and Inflammation Response

Figure 4

Knockdown of TREM1 suppresses the inflammatory response and NF-κB signaling activation in LPS-induced intestinal epithelium cells. (a) Representative images of western blot results for TREM1, p-p65, ad p-IκBa in LPS-induced intestinal epithelium-6 (IEC-6) cells transfected with TREM1 specific siRNA oligos or negative control oligos. (b–d) The expression levels of (b) TREM1 (), (c) p-p65 (), and (d) p-IκBa () were quantified in the indicated groups. (e–g) The mRNA expression levels of (e) TNF-α (), (f) IL-6 (), and (g) IL-1β () were evaluated by RT-qPCR. for each group. One-way analysis of variance (ANOVA) was used for multiple comparisons, followed by Tukey’s test. ns: no statistical significance; ; .

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