Assessment of Successful qRT-PCR of SARS-CoV-2 Assay in Pool Screening Using Isopropyl Alcohol Purification Step in RNA Extraction

Table 1

SARS-CoV-2 novel coronavirus primers and probes for real-time RT-PCR diagnosis.

Assay/use	Oligonucleotide ID	Sequence (5–3)
E gene	E_Sarbeco_F1	ACAGGTACGTTAATAGTTAATAGCGT
	E_Sarbeco_R2	ATATTGCAGCAGTACGCACACA
	E_Sarbeco_P1	FAM-ACACTAGCCATCCTTACTGCGCTTCG-BHQ
RNase P gene (internal control)	RNase P forward	AGATTTGGACCTGCGAGCG
	RNase P reverse	GAGCGGCTGTCTCCACAAGT
	RNase P probe	FAMTTCTGACCTGAAGGCTCTGCGCGBHQ
RdRp gene	RdRP_SARSr-F2	GTGARATGGTCATGTGTGGCGG
	RdRP_SARSr-R1	CARATGTTAAASACACTATTAGCATA
	RdRP_SARSr-P2 specific for Wuhan-CoV	FAM-CAGGTGGAACCTCATCAGGAGATGC-QSY
HKU ORF gene	HKU-ORF1b-nsp14F	TGGGGYTTTACRGGTAACCT’
	HKU-ORF1b-nsp14 R	AACRCGCTTAACAAAGCACTC
	HKU-ORF1b-nsp14 P	FAM-TAGTTGTGATGCWATCATGACTAG-QSY

R is G/A; FAM: 6-carboxyfluorescein; BHQ: Black Hole Quencher; QSY Quencher (select quencher none in plate set up).