Ginkgo Biloba Extract EGB761 Ameliorates the Extracellular Matrix Accumulation and Mesenchymal Transformation of Renal Tubules in Diabetic Kidney Disease by Inhibiting Endoplasmic Reticulum Stress

Figure 2

EGB761 reduced EMT in DKD. (a) The protein expression of α-SMA, E-cadherin, and bar graph representing the quantification of the α-SMA and E-cadherin bands in the renal tissue of each mice group. (b) The mRNA expression of α-SMA and E-cadherin in the renal tissue. (c) The protein expression of α-SMA, E-cadherin, and bar graph representing the quantification in HK-2 cells. (d) The mRNA expression of α-SMA and E-cadherin in HK-2 cells. (e) Immunofluorescent of α-SMA. (f) Immunofluorescent of E-cadherin. Ctrl: normal group or treated with PBS; DKD: diabetic kidney disease group; DKD + EGB761: diabetic kidney disease group+EGB761 (36 mg/kg); DKD+4-PBA: diabetic kidney disease group+4-phenylbutyrate (50 g·L-1, 1 g·kg-1); HG: high glucose; HG + HD: high glucose+EGB761 40 mg/L; HG + MD: high glucose+EGB761 20 mg/L; HG + LD: high glucose+EGB761 10 mg/L. Results were expressed as . , versus Ctrl. ^#, ^## versus DKD model group or HG.

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