Ginkgo Biloba Extract EGB761 Ameliorates the Extracellular Matrix Accumulation and Mesenchymal Transformation of Renal Tubules in Diabetic Kidney Disease by Inhibiting Endoplasmic Reticulum Stress

Figure 3

EGB761 reduced ECM accumulation in DKD. (a) The protein expression of collagen IV, fibronectin, and bar graph representing the quantification in the renal tissue. (b) The mRNA expression of collagen IV and fibronectin in the renal tissue. (c) The protein expression of collagen IV, fibronectin, and bar graph representing the quantification in HK-2 cells. (d) The mRNA expression of collagen IV and fibronectin in HK-2 cells. (e) Immunofluorescent of collagen IV. (f) Immunofluorescent of fibronectin. Ctrl: normal group or treated with PBS; DKD: diabetic kidney disease group; DKD + EGB761: diabetic kidney disease group+EGB761 (36 mg/kg); DKD+ 4-PBA: diabetic kidney disease group+4-phenylbutyrate (50 g·L-1, 1 g·kg-1); HG: high glucose; HG + HD: high glucose+EGB761 40 mg/L; HG + MD: high glucose+EGB761 20 mg/L; HG + LD: high glucose+EGB761 10 mg/L. Results are expressed as . , versus Ctrl. ^#, ^## versus DKD model group or HG.

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