Ginkgo Biloba Extract EGB761 Ameliorates the Extracellular Matrix Accumulation and Mesenchymal Transformation of Renal Tubules in Diabetic Kidney Disease by Inhibiting Endoplasmic Reticulum Stress

Figure 4

EGB761 inhibited ERS in DKD. (a) Immunohistochemical of GRP78, ATF6 in each mice group. (b) The protein expression of GRP78 and ATF6, as well as bar graph representing the quantification of the GRP78 and ATF6 bands in the renal tissue. (c) The mRNA expression of GRP78 and ATF6 in the renal tissue. (d) The protein expression of GRP78 and ATF6, as well as bar graph representing the quantification in HK-2 cells. (e) The mRNA expression of GRP78 and ATF6 in HK-2 cells. Ctrl: normal group or treated with PBS; DKD: diabetic kidney disease group; DKD + EGB761: diabetic kidney disease group+EGB761 (36 mg/kg); DKD+4-PBA: diabetic kidney disease group+4-phenylbutyrate (50 g·L-1, 1 g·kg-1); HG: high glucose; TM: tunicamycin; HG + MD: high glucose+EGB761 20 mg/L; TM + MD: tunicamycin+EGB761 20 mg/L. Results are expressed as . , versus Ctrl. #, ## versus DKD model group or HG. ▲, ▲▲ versus TM.

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