Research Article

Selection and Evaluation of Potential Reference Genes for Quantitative Real-Time PCR in Agaricus blazei Based on Transcriptome Sequencing Data

Table 2

Selected candidate reference genes, primers, and amplification characteristics derived from qRT-PCR analysis.

GeneA. blazei gene IDDescriptionPrimer sequence (5-3)Product size (bp)Amplification efficiency (%)

Uqcr8A08809Ubiquinol-cytochrome c reductase complex subunit 8F:CACCCAATATGCCATTTCTCC
R:AATCCCGTATCCGACACCAA
13699.21.000
Vps26A09130Vacuolar protein sorting 26F:GGGAGAAGATGCTCGGAAAC
R:CCGCCAACTCTTGCGATAA
23090.00.998
Gnb2A04205Guanine nucleotide-binding protein beta subunit 2F: ACAAAGTCCACGCCATCCCT
R: GAGACCACCGCAAGCAACAT
8992.90.998
Copb1A08816Coatomer subunit betaF:TTATTGGGAAGTCTGTCCGAAGT
R: TGAACGACAGGTCGGTATGAG
19093.70.998
UqcrcA01805Core subunit of the ubiquinol-cytochrome c reductase complexF: ACAACCTCGCCAACTCATTCA
R:TGCTCATTCTTGCCCATTCTTT
141101.40.997
ATP7A05732ATP7 subunit D of the stator stalk of mitochondrial F1F0 ATP synthaseF: TCGCTCATTATCGCACTATCCT
R:GGCACTGTCCATTTACCCTTCTT
31993.70.999
NdufS7A01315NdufS7 ubiquinone oxidoreductase subunitF: CCTTGAAGTCCGCAGTTTGTAATG
R: TTTGTTTGTGGAGACGAGGTGAAT
10097.60.998
PUP3A0428020S proteasome subunit beta 3F:GAAGAGCGTGAGATTGAACCAGA
R:TCTGTCGCAGCAATAAAGGGTT
15295.20.996
PsmB6A05770Proteasome subunit beta type-6F: CGTCCACGACCGCATTTACT
R:AGGTCCACCCGACATTTGC
11296.80.999
RasA03682Ras proteinF:GGCAACAAGTGCGACAAATCA
R:CCACAGCGAAAGCAACAGAAA
251103.40.997
CPR1A01938Probable proteasome subunit beta type-4F: CCTGCTGCTGCCTCGTCTT
R:CTCTTGAGTGTTTCTATGCCTTCC
26092.70.996
CSA02079Citrate synthaseF:GCTTGTCGGTTCAGCCCTTTCT
R:TCTTCCTTAGAACGGCGTGGC
23097.40.997
SPRYpA04044Endosomal SPRY domain proteinF: ACCCTATCCCAGTTCCTTTCCA
R:CTTCACCTCCCAGTAGTAGACTTCATT
198103.00.998
GAPDHA07487Glyceraldehyde-3-phosphate dehydrogenaseF: TTCGGCTCATTTGAAGGGTG
R:TTGGTTGTGCAAGAAGCGTTAG
14094.00.998
ATP2A06017ATP2 beta subunit of the F1 sector of mitochondrial F1F0 ATP synthaseF:CCGAATACTTCCGTGACGAGG
R:TTGGTAACCGACAGCAGAGGG
12899.00.999
ATP1A07930ATP1 alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthaseF: TCCCTCCTTCTCCGTCGTCC
R:TCTTAGTCTGGGCGGCTGAA
30096.60.999
Cdc4A08640Myosin regulatory light chain cdc4F: CAGGCAGAAGTCGCTGAGATTGT
R:GAGGATTGTGCGGACAAAGGAT
30096.30.992
FKS1A098271, 3-beta-glucan synthaseF: ACCATTCACGCCGATTACATT
R:GCATAGCATTACGCCAACGA
187104.00.996
Cox5aA03337COX5A subunit VA of cytochrome c oxidaseF: TCTCGTCCGTTGTGCGTCTG
R:TCGCGGTAGCGTAGTCGTTGA
20693.20.996
ATPDHA06239ATP-dependent helicaseF: TCAAATCTGGCGTTGTCCC
R:CCGATTCCTTGATGTTCATTACTC
211104.30.986
α-TUBA05905Tubulin alphaF:CATTTCCCACTTGCCACTTTC
R:CAACAGCAGCATTGACATCCTTA
202100.30.999
NdufA2A00266NdufA2, NADH ubiquinone oxidoreductase 10.5kD subunitF: GCGAACTCCGTTTCTTCTTCTCCC
R:AGTCTGGGTTGTGCTGTTTGATGG
10296.20.994
RPT6A0169826S proteasome regulatory complex ATPase RPT6F: GTTGTTTGTTATGGCTCGTGAA
R:AAGCAACTCCAACATCGTCCT
13696.30.993
ATP3A08690ATP3 gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthaseF: ACAAGTTCGTTTCTGCCATCTCC
R:TGGTAGCATCATCCTCGTTCTCAT
11497.80.999
NDUFB9A09944NDUFB9 NADH-ubiquinone oxidoreductase complex IF:TACCTCACAAACTCGCTTGACTGG
R:CGGTTACATCACGGTTCTTCTCG
10092.80.994
β-TUB2A00521Tubulin beta-2F:CGGGCAAGATGTAGAGCAC
R:TCCAGGATAAAAGGCGAAG
231100.80.996
STT3A07729Oligosaccharyl transferase STT3 subunitF: TGTAGCCAACACTGCCTCCT
R:TGCCAAAAGCACACAAATG
33198.50.997

F and R indicated forward primer and reverse primer, respectively; referred to coefficient of determination.