Research Article

Genome Features and In Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains

Table 1

The main genome characteristics of the studied probiotic bacteria.

BacteriaMain characteristics of the genomeDeterminants of bacteriocin synthesisDeterminants of antibiotic resistanceDeterminants of sugar metabolismDeterminants of exopolysaccharide synthesis

L. plantarum 8 RA 3GenBank number LBDF00000000
Genome type draft
Number of contigs 18
Genome size 3,330,093 bp
Average coverage 250.0
GC composition 44.4%
CDS quantity 2982
KLD40925.1, KLD40929.1, KLD40930.1, KLD40931.1, KLD40932.1, KLD40933.1
Contig 4 LBDF01000008.1
Efflux pumps
MatE
KLD43042.1
MFS
KLD43077.1
426 determinants
Fructose bisphosphate pathway
Pentose phosphate pathway
Utilization of fructooligosaccharides and raffinose: Msm KLD40778.1, PTS KLD43366.1, KLD43367.1, KLD43368.1, SacA KLD43120.1, DexB KLD43365.1, Aga KLD42863.1, Man KLD43356.1 и BG KLD41117.1
Utilization of amino sugars: NagA KLD43035.1, NagB KLD43152.1, PTS NagT KLD42190.1, NagR KLD40894.1, Cbp KLD42577.1 ChiA KLD43348.1
Glycogen metabolism: GAT KLD43421.1, GS KLD43423.1, GP KLD43424.1
Eps operon
EpsB KLD41507.1
EpsC KLD40740.1
EpsD KLD40739.1
EpsE KLD41508.1
EpsF KLD41509.1
Contig 15
LBDF01000011.1

L. fermentum 90 TC-4GenBank number
LBDH00000000
Genome type draft
Number of contigs 93
Genome size 1,822,484 bp
Average coverage 250.0
GC composition 51.9%
CDS 1620 quantity
Efflux pumps
MatE
KLD54124.1
MFS
KLD53990.1
113 determinants
Pentose phosphate
Pathway utilization of disaccharides: GalK KLD53103.1, FruK KLD55067.1, α-Gal KLD53100.1, β-Gal WP_046949162.1, mapA P_046948545.1, β-glucosidase KLD43584.1
Eps operon
EpsB KLD49590.1
EpsC KLD49588.1
EpsD KLD49589.1
Contig 15
LBDH01000061.1

L. fermentum 39GenBank number LBDG00000000
Genome type draft
Number of contigs 55
Genome size 1,829,655 bp
Average coverage 250.0
GC composition 51.6%
CDS quantity 1683
Efflux pump
MatE
KLD56150.1
118 determinants
Pentose phosphate metabolic pathway
Utilization of arabinose: AraR KLD56054.1, AraK KLD56053.1, AraA KLD56052.1, AraD KLD56052.1, AraT KLD56053.1
Glycerol utilization: GlpT KLD54015.1, GlpF KLD56071.1, GlpK KLD55877.1, GPD KLD55407.1
Riboflavin metabolism: RybD KLD54636.1, RSA KLD54605.1
Eps operon
EpsB KLD51897.1
EpsC KLD51899.1
EpsD KLD51898.1
EpsE KLD51903.1
Contig 20
LBDG01000035.1

B. longum 379GenBank number LKUQ00000000
Genome type draft
Number of contigs 24
Genome size 2,387,620 bp
Average coverage 150.0
GC composition 60.2%
CDS 1903 quantity
Efflux pump
MatE
KYJ82530.1
цитоплазматический белок tetW KYJ81078.1
199 determinants
Fructose-6-phosphate phosphoketolase pathway
Amino sugar metabolism: NagK KYJ78240.1, NagA KYJ78237.1, NagB KYJ78238.1, NagT KYJ81992.1, NagR KYJ82080.1
Glycogen metabolism: GAT KYJ81114.1, GS KYJ81082.1, GBr KYJ82453.1, GP KYJ83271.1, GdBr KYJ82081.1, AMse KYJ82084.1, MalE KYJ78457.1
Utilization of raffinose and fructooligosaccharides: MsmR KYJ82093.1, MsmE KYJ82142.1, MsmF KYJ82092.1, MsmG, KYJ82141.1, SacA KYJ78008.1, Aga KYJ83465.1
Genes for rhamnose synthesis, contig 5 LKUQ01000020.1
Capsular polysaccharide genes: Wzb KYJ83195.1, Wzc KYJ83223.1
Sortase dependent pili: SrtA KYJ83477, AP KYJ83476.1
Lipoproteins: Lgt KYJ83617.1, LspA KYJ77995.1

B. bifidum 1GenBank NDXI00000000
Genome type draft
Number of contigs 13
Genome size 2,198,027 bp
Average coverage 385.0
GC composition 62.7%
CDS 1521 quantity
Efflux pump
MatE
PDH98462.1
175 determinants
Fructose-6-phosphate phosphoketolase pathway
Amino sugar metabolism: NagK PDH98478.1, NagA PDH97531.1, NagB PDH98129.1, NagT PDH97280.1, CbsA PDH98222.1, Aga PDH97428.1
Sortase dependent pili: SrtA PDH97100.1, PDH97310.1
Lipoproteins: Lgt PDH98440.1, LspA PDH98074.1

B. bifidum 791GenBank number LKUR00000000
Genome type draft
Number of contigs 33
Genome size 2,285,457 bp
Average coverage 150.0
GC composition 62.4%
CDS 1769 quantity
Efflux pumps
MatE
KYJ84349.1, KYJ84414.1
207 determinants
Fructose-6-phosphate phosphoketolase pathway
Amino sugar metabolism: NagK KYJ84330, NagA KYJ85076.1, NagB KYJ85077.1, NagT KYJ85215.1, KYJ85216.1, NagR KYJ85078.1, CbsA KYJ83728.1, Aga KYJ84485.1
Glycogen metabolism: GAT KYJ83153.1, GS KYJ84446.1, GBr KYJ84933.1, GP KYJ84691.1, GdBr KYJ85154.1, AMse KYJ84544.1
Genes for the synthesis of rhamnose, contig 3 LKUR01000023.1
Sortase dependent pili: SrtA KYJ84870.1, AP KYJ84871.1
Lipoproteins: Lgt KYJ84380.1, LspA KYJ85145.1