Differentially Expressed Long Noncoding RNAs Involved in FUBP1 Promoting Hepatocellular Carcinoma Cells Proliferation

Figure 5

Inhibition of lnc-LYZ-2 alleviate progrowth effect of FUBP1 on HCC cells. (a) Quantitative RT-PCR assay proved that lnc-LYZ-2 expression was obviously increased in HCC cells infected byLv-FUBP1 but decreased after of lnc-LYZ-2 inhibition. vs. the Lv-GFP group and ^## vs. the Lv-FUBP1 group. (b) CCK-8 assay showed the growth vitality of HCC cells transfected with si-lnc-LYZ-2 was significantly reduced on FUBP1-overexpression condition. vs. the Lv-FUBP1+si-NC group. (c) CCK-8 assay showed that inhibition of lnc-LYZ-2 decreased the growth vitality of MHCC97-L cell lines on normal condition but had no effect on MHCC97-H and Huh7 HCC cell lines. vs. the si-NC group. (d) Quantitative RT-PCR assay proved that lnc-LYZ-2 expression was obviously increased in HCC cells infected by Lv-lnc-LYZ-2. vs. the Lv-GFP group. (e) CCK-8 assay showed that overexpression of lnc-LYZ-2 increased the growth vitality of HCC cell lines. vs. the Lv-GFP group.

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