Quantitative Phosphoproteomic Comparison of Lens Proteins in Highly Myopic Cataract and Age-Related Cataract

Table 1

Phosphosites differentially expressed in HMC-C vs HMC-N.

Protein names	Gene name	Accession	Score	Modified sequence	Position	FC	value
Phosphosites exclusively detected in HMC-C
Alcohol dehydrogenase 1A	ADH1A	P07327	106.43	_EIGADLVLQIS(ph)KESPQEIAR_	225	NaN	0.003
Alcohol dehydrogenase 1A	ADH1A	P07328	105.84	_S(ph)GGTLVLVGLGSEMTTVPLLHAAIR_	266	NaN	0.039
Aldehyde dehydrogenase family 1 member A3	ALDH1A3	P47895	88.19	_IFINNEWHES(ph)KSGK_	43	NaN	0.026
Filensin	BFSP1	Q12934	73.156	_S(ph)RS(ph)LPEKGPPK_	605	NaN	0.003
Filensin	BFSP1	Q12934	97.214	_VLEKS(ph)SYDCR_	288	NaN	0.034
Beta-crystallin B2	CRYBB2	P43320	94.45	_DSSDFGAPHPQVQS(ph)VRR_	186	NaN	0.010
Protein 4.1	EPB41	P11171	63.16	_KLS(ph)MYGVDLHK_	394	NaN	0.013
Glutathione reductase	GSR	P00390	74.12	_GHAAFT(ph)SDPKPTIEVSGK_	177	NaN	0.008
Phakinin	BFSP2	Q13515	102.6	_MDLESQIESLKEELGSLS(ph)R_	238	NaN	0.011
Galectin-1	LGALS1	P09382	74.12	_SFVLNLGKDS(ph)NNLCLHFNPR_	39	NaN	≤0.001
Leukotriene A-4 hydrolase	LTA4H	P09960	94.85	_CS(ph)VDFTR_	27	NaN	0.002
Mpv17-like protein 2	MPV17L2	Q567V2	106.13	_LLS(ph)AGQLLFQGR_	14	NaN	0.004
Neurofascin	NRCAM	O94856	78.342	_SGT(ph)LVIDFR_	98	NaN	0.001
Peroxiredoxin-6	PRDX6	P30041	50.364	_RVATPVDWKDGDS(ph)VMVLPTIPEEEAK_	186	NaN	0.005
cAMP-dependent protein kinase catalytic subunit alpha	PRKACA	P17612	68.108	_KGS(ph)EQESVK_	11	NaN	0.003
Ribose-phosphate diphosphokinase	PRPS2	A0A140VK41	53.865	_VAILVDDMADT(ph)CGTICHAADK_	228	NaN	≤0.001
Tryptophan-tRNA ligase, cytoplasmic	WARS	P23381	99.802	_KLS(ph)FDFQ_	467	NaN	≤0.001
Phosphosites exclusively detected in HMC-N
Carbonic anhydrase 4	CA4	P22748	69.03	_EQT(ph)VSM(ox)KDNVR_	263	NaN	0.001
Carbonyl reductase (NADPH) 1	CBR1	P16152	75.462	_LFS(ph)GDVVLTAR_	30	NaN	≤0.001
Beta-crystallin A4	CRYBA4	P53673	60.564	_GEY(ph)PSWDAWGGNTAYPAER_	74	NaN	≤0.001
Glutathione synthetase	GSS	P48637	156.67	_QIEINTIS(ph)ASFGGLASR_	149	NaN	0.003
HSPC141	PHPT1	Q9P019	66.871	_(ac)AVADLALIPDVDIDS(ph)DGVFK_	16	NaN	0.001
Phosphosites with upregulation in HMC-C (selected)
S-Formylglutathione hydrolase	ESD	P10768	117.37	_MSIFGHS(ph)MGGHGALICALK_	149	4.61	0.003
Glyceraldehyde-3-phosphate dehydrogenase	GAPDH	P04406	67.995	_IISNASCT(ph)TNCLAPLAK_	153	2.58	0.001
Filensin	BFSP1	Q12934	103.27	_LQLEAQFLQDDIS(ph)AAKDR_	170	2.57	0.037
Alpha-crystallin A chain	CRYAA	P02489	123.19	_QS(ph)LFRTVLDSGISEVR_	51	2.41	0.003
Fructose-bisphosphate aldolase	ALDOA	J3KPS3	80.229	_CQY(ph)VTEK_	208	2.39	0.023
Phosphosites with downregulation in HMC-C
Gamma-crystallin C	CRYGC	P07315	125.77	_RGEYPDYQQWM(ox)GLS(ph)DSIR_	73	0.02	0.036
Fructose-bisphosphate aldolase	ALDOA	J3KPS3	132.4	_GILAADEST(ph)GSIAKR_	37	0.15	≤0.001
Beta-crystallin A3	CRYBA1	P05813	184.26	_VES(ph)GAWIGYEHTSFCGQQFILER_	70	0.45	0.031
Gamma-crystallin C	CRYGC	P07315	235.83	_VES(ph)GCWMLYERPNYQGQQYLLR_	40	0.48	0.029