Quantitative Phosphoproteomic Comparison of Lens Proteins in Highly Myopic Cataract and Age-Related Cataract

Table 2

Phosphosites differentially expressed in ARC-C vs ARC-N.

Protein names	Gene name	Accession	Score	Modified sequence	Position	FC	value
Phosphosites exclusively detected in ARC-C
Actin, cytoplasmic 1	ACTA2	P60709	111.6	_GYS(ph)FTTTAER_	199	NaN	0.006
Alcohol dehydrogenase 1A	ADH1A	V9HW89	93.73	_SGGTLVLVGLGS(ph)EMTTVPLLHAAIR_	277	NaN	0.024
Alcohol dehydrogenase 1A	ADH1A	V9HW89	82.89	_EIGADLVLQISKES(ph)PQEIAR_	228	NaN	≤0.001
Alcohol dehydrogenase 1A	ADH1A	V9HW89	105.8	_S(ph)GGTLVLVGLGSEMTTVPLLHAAIR_	266	NaN	0.001
Cysteine protease	ATG4D	B4DZK0	64.3	_KYS(ph)IFTEKDEILSDVASR_	151	NaN	0.001
Alpha-crystallin A chain	CRYAA	P02489	142.5	_HFSPEDLT(ph)VK_	86	NaN	≤0.001
Beta-crystallin A2	CRYBA2	P53672	85.46	_LLS(ph)DCANVCER_	31	NaN	0.026
Beta-crystallin A4	CRYBA4	P53673	86.48	_GFQYVLECDHHS(ph)GDYK_	170	NaN	0.015
Beta-crystallin B1	CRYBB1	P53674	162.4	_WNTWSS(ph)SYR_	129	NaN	0.016
Quinone oxidoreductase	CRYZ	Q08257	98.77	_AGESVLVHGAS(ph)GGVGLAACQIAR_	158	NaN	≤0.001
Eukaryotic initiation factor 4A-II	EIF4A2	Q14240	137.9	_GYDVIAQAQS(ph)GTGK_	79	NaN	≤0.001
Protein 4.1	EPB41	P11171	99.02	_QAS(ph)ALIDRPAPHFER_	521	NaN	≤0.001
Glucose-6-phosphate isomerase (fragment)	GPI	A0A0A0MTS2	65.72	_ELQAAGKS(ph)PEDLER_	470	NaN	≤0.001
Heat shock 70 kDa protein 4	HSPA4	P34932	63.71	_AFS(ph)DPFVEAEK_	76	NaN	≤0.001
Glutathione synthetase	GSS	P48637	68.94	_DGY(ph)MPRQYSLQNWEAR_	270	NaN	0.003
Phakinin	BFSP2	Q13515	110.9	_AAEEEINS(ph)LYK_	208	NaN	≤0.001
Phakinin	BFSP2	Q13515	58.69	_VHALEQVSQELET(ph)QLR_	134	NaN	0.006
Lactase-like protein	LCT	Q6UWM7	86.29	_S(ph)AEQGLEM(ox)SR_	311	NaN	≤0.001
L-Lactate dehydrogenase A chain	LDHC	P00338	90.61	_S(ph)ADTLWGIQK_	319	NaN	0.028
Neurofascin	NRCAM	O94856	78.34	_SGT(ph)LVIDFR_	98	NaN	≤0.001
Protein kinase C and casein kinase substrate in neurons 3	PACSIN3	D3DQR0	92.54	_LKEVEAS(ph)K_	153	NaN	0.001
Peroxisome biogenesis factor 10, isoform CRA_b	PEX10	A0A024R0A4	87.26	_RAS(ph)LEER_	281	NaN	0.006
Phosphoglycerate mutase 1	PGAM1	P18669	56.72	_FSGWYDADLS(ph)PAGHEEAKR_	31	NaN	0.016
Plectin	PLEC	Q15149	93.16	_LS(ph)FSGLR_	3441	NaN	≤0.001
Plectin	PLEC	Q15149	69.72	_KAS(ph)DSELER_	2039	NaN	≤0.001
Plectin	PLEC	Q15149	78.65	_KES(ph)YSALMR_	794	NaN	0.007
cAMP-dependent protein kinase catalytic subunit alpha	PRKACA	P17612	68.11	_KGS(ph)EQESVK_	11	NaN	≤0.001
Ribose-phosphate diphosphokinase	PRPS2	A0A140VK41	53.87	_VAILVDDMADT(ph)CGTICHAADK_	228	NaN	≤0.001
Glycogen phosphorylase	PYGB	P06737	61.64	_RMS(ph)LIEEEGSKR_	430	NaN	0.002
SEC14-like protein 2	SEC14L2	O76054	122.5	_VGDLS(ph)PR_	9	NaN	≤0.001
Tryptophan-tRNA ligase, cytoplasmic	WARS	P23381	99.8	_KLS(ph)FDFQ_	467	NaN	0.002
Synaptobrevin homolog YKT6	YKT6	O15498	53.03	_IDWPVGS(ph)PATIHYPALDGHLSR_	114	NaN	0.010
14-3-3 protein	YWHAH	Q04917	51.15	_KNS(ph)VVEASEAAYK_	145	NaN	≤0.001
Phosphosites exclusively detected in ARC-N
Nucleoside triphosphate pyrophosphatase	ASMT	O95671	80.98	_VVLASAS(ph)PR_	21	NaN	≤0.001
Alpha-crystallin B chain	CRYAA	P02511	127	_LFDQFFGEHLLESDLFPTSTSLS(ph)PFYLRPPSFLR_	45	NaN	0.010
Crystallin gamma B	CRYGB	A0A0U3BWM0	73.78	_GQMSELT(ph)DDCLSVQDR_	107	NaN	0.004
3-Hydroxyanthranilate 3,4-dioxygenase	HAAO	P46952	78.43	_RLS(ph)LAPDDSLLVLAGTSYAWER_	247	NaN	0.007
Phosphosites with upregulation in ARC-C (selected)
Beta-crystallin A3	CRYBA1	P05813	69.26	_ITIY(ph)DQENFQGK_	36	####	≤0.001
Alpha-crystallin B chain	CRYAA	P02511	177.9	_RPFFPFHSPS(ph)R_	21	####	0.049
Phakinin	BFSP2	Q13515	174.6	_SS(ph)SSLES(ph)PPASR_	38	####	≤0.001
Alpha-crystallin B chain	CRYAA	P02511	151.2	_LEKDRFS(ph)VNLDVK_	76	####	0.001
Filensin	BFSP1	Q12934	173.6	_VRS(ph)PKEPETPTELYTK_	454	####	0.009
Phosphosites with downregulation in ARC-C
Beta-crystallin A3	CRYBA1	P05813	252.2	_RMEFTS(ph)SCPNVSER_	50	0.26	0.005
Gamma-crystallin D	CRYGD	P07320	151.2	_RGDYADHQQWMGLS(ph)DSVR_	73	0.27	0.009
Coactosin-like protein	COTL1	Q14019	70.09	_FTTGDAMS(ph)KR_	71	0.45	≤0.001
Beta-crystallin A3	CRYBA1	P05813	123.4	_WDAWS(ph)GSNAYHIER_	100	0.50	0.049