Research Article

Differences in the Expression of KIR, ILT Inhibitory Receptors, and VEGF Production in the Induced Decidual NK Cell Cultures of Fertile and RPL Women

Table 1

Mouse anti-human antibodies with conjugated fluorochromes were used in the experiment. Phenotyping was performed in 4 color schemes in three tubes. Each tube included CD56 and CD16 to gate the population of interest: in tube 1, the induction of NK cells to idNK cells and killer immunoglobulin-like receptors were assessed, and in tube 2, ILT expression was checked.

MarkerFluorochromeCloneIsotype controlManufacturerThe volume of Abs per tubeTubes

CD56PE-Cy7B159Becton Dickinson5 μl1, 2
CD16FITC3G8Becton Dickinson20 μl1, 2
CD158a/KIR2DLPEHP-3E4Mouse BALB/c IgMBecton Dickinson20 μl1
CD159a/NKG2AAPCZ199Mouse IgG2bBeckman Coulter7 μl1
CD85j/ILT-2PEGHI/75Mouse IgG2bBecton Dickinson20 μl2
CD85d/ILT-4Alexa647287219Mouse IgG2a, κBecton Dickinson5 μl2