miR-125a-5p Regulates Osteogenic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells under Oxidative Stress

Figure 5

miR-125a-5p mediates the VEGF-regulated osteogenic differentiation of hADSCs under oxidative stress. (a) The expression level of VEGF protein in hADSCs infected by miRNA-125a-5p or infected with miRNA-125a-5p inhibitor was measured by western blotting. (b) The expression level of VEGF was evaluated at different time points during osteoinduction with or without H₂O₂. (c) The expression level of VEGF was measured by qRT-PCR on day 21, and after that, the cells were induced by the osteogenic differentiation medium (ODM) and, with the H₂O₂ stimulation, infected by miRNA-125a-5p or infected with miRNA-125a-5p inhibitor. ; ^ns; .

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