Canine Natural Killer Cell-Derived Exosomes Exhibit Antitumor Activity in a Mouse Model of Canine Mammary Tumor

Figure 1

Isolation and characterization of canine NK cells. (a) CD5 negative (CD5^lo) cells were isolated from canine PBMCs and activated in specific culture supplemented with human IL-2 (500 U/mL), IL-15 (10 ng/mL), and canine IL-21 (5 ng/mL) for 21 days. (b) Cell surface markers were analyzed by FACS flow cytometric analysis at activated CD5^lo cells. (c) To determine whether CD5^lo cells develop into cytotoxic NK cells, the expression of activated NK cell markers (NKp30, NKp44, NKp46, NKG2D, CD244, Perforin 1, and Granzyme B) in activated canine CD5^lo cells was analyzed by quantitative RT-PCR. β-Actin was used as a reference gene. , , .

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