RBM23 Drives Hepatocellular Carcinoma by Activating NF-κB Signaling Pathway

Figure 2

RBM23 promotes tumor angiogenesis in vitro. (a) Wound healing assay showing that supernatant from the Huh-7-ov-RBM23 cells highly promoted the migration of HUVECs in vitro compared to the corresponding cells. The stimulating effect of the medium from HepG2/-sh-RBM23 on the migration of HUVECs was not detectable. (b) Cell counting kit-8 assays showing RBM23 knockdown in HepG2 cells inhibited HUVEC growth whereas RBM23 overexpression in Huh-7 cells promoted HUVEC growth. (c) Images of Matrigel plugs retrieved, and there are significantly more blood vessels in plugs mixed with the cell supernatant of the overexpression group. (d) The mRNA levels of RelA, IL-6, IL-8, and VEGF from different cells as determined by real-time PCR analysis.

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