[Retracted] Pentoxifylline Can Reduce the Inflammation Caused by LPS after Inhibiting Autophagy in RAW264.7 Macrophage Cells

Figure 3

The content of IL-1β and TNF-α in supernatants was examined with ELISA. RAW264.7 cells were firstly treated with 0.2 mg/mL PTX (a) and 0.5 mg/mL (b) for 12 h, respectively, and then treated with 100 ng/mL LPS and 20 ng/mL IFN-γ. After 24 h incubation, carefully remove the medium without disturbing the layer of cells and collect the supernatant for ELISA analysis (; ^##; ^###). (c) The influences of pretreatment of PTX on the expression of IL-1β and TNF-α in RAW264.7 cells. The cells were firstly treated with 0.5 mg/mL of PTX for 6, 12, and 24 h, respectively. Then, cells were treated with 100 ng/mL LPS and 20 ng/mL IFN-γ for 24 h. After that, the cellular proteins were collected and examined by Western blotting. (d) The effects of PTX pretreatment concentration on protein expression of IL-1β and TNF-α in RAW264.7 cells. The cells were pretreated with 0.2, 0.3, and 0.5 mg/mL PTX for 12 h, respectively, and then treated with 100 ng/mL LPS and 20 ng/mL IFN-γ for 24 h. After that, the cellular proteins were collected and detected by Western blotting. Both in (c) and (d) the α-tubulin was the internal control, and quantitative analyses of protein expressions are represented by a bar graph (; ^###).

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