MicroRNA-210-3p Promotes Chondrogenic Differentiation and Inhibits Adipogenic Differentiation Correlated with HIF-3α Signalling in Bone Marrow Mesenchymal Stem Cells

Figure 4

miR-210 directly regulates HIF-3α. (a) Binding sites of miR-210-3p and HIF-3α. (b) miR-210 mRNA levels were measured after transfection with HIF-3α siRNA or the control group. (c) HIF-3α protein levels were measured after transfection with HIF-3α siRNA or the control group. GAPDH was the internal control. (d) HIF-3α mRNA levels were measured after transfection of the three groups. (e) HIF-3α protein levels were measured after transfection with normal, miR-210 mimic, and miR-210 inhibitor groups. (f) PPARγ and LPL protein levels were measured after transfection with the miR-210 inhibitor group and the miR-210 inhibitor+HIF-3α siRNA group. (g) COLII and SOX9 protein levels were measured after transfection with the miR-210 inhibitor group and the miR-210 inhibitor+HIF-3α siRNA group (, ).

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