Prevalence of Trypanosoma congolense and Trypanosoma vivax in Lira District, Uganda

Table 1

ITS1 primer sequence and PCR conditions.

Primer name	Primer sequence	Trypanosomes species	Fragment size (bp)	Melting temperature
ITS1 CF	F 5-CCGGAAGTTCACCGATATTG-3	T. congolense savannah	700	Denaturation at 98°C
			700
	R 5-TTGCTGCGTTCTTCAACGAA-3 [23]	T. congolense forest	620	Annealing at 64°C
			250	Extension at 72°C
		T. congolense kilifi	300
		T. vivax	400
		T. godfreyi
		T. simiae