Research Article

UPLC-ESI-MS/MS Analysis and Evaluation of Antioxidant Activity of Total Flavonoid Extract from Paeonia lactiflora Seed Peel and Optimization by Response Surface Methodology (RSM)

Table 1

Box-Behnken design and response values for the flavonoid yield of P. lactiflora seed peel.

Run: ethanol concentration: liquid-solid ratio: ultrasonic time: flavonoid yield
(%)(mL/g)(min)(mg RE/g)

170 (1)30 (1)60 (0)10.206
270 (1)25 (0)50 (-1)9.642
360 (0)25 (0)60 (0)11.202
450 (-1)20 (-1)60 (0)8.919
570 (1)25 (0)70 (1)9.956
660 (0)20 (-1)50 (-1)9.053
770 (1)20 (-1)60 (0)9.633
860 (0)20 (-1)70 (1)9.156
960 (0)25 (0)60 (0)10.984
1060 (0)30 (1)70 (1)9.548
1150 (-1)25 (0)50(-1)9.556
1250 (-1)25 (0)70 (1)9.316
1360 (0)25 (0)60 (0)11.038
1460 (0)30 (1)50 (-1)9.636
1550 (-1)30 (1)60 (0)10.002
1660 (0)25 (0)60 (0)11.068
1760 (0)25 (0)60 (0)11.016