UPLC-ESI-MS/MS Analysis and Evaluation of Antioxidant Activity of Total Flavonoid Extract from Paeonia lactiflora Seed Peel and Optimization by Response Surface Methodology (RSM)

Table 3

The content of flavonoids in the extract of P. lactiflora seed peel was determined by UPLC-ESI-MS/MS (ng/g of extract).

Peak	RT (min)	Identification	Ethanol extract	Q1 ()	Q3 ()	CE
1	8.42	Protocatechuic acid	186217.33	152.9	108.9	-20
2	10.3	3,4-Dihydroxybenzaldehyde	32983.74	307	139.3	20
3	10.54	Gentisic acid	5491.66	310.9	148.9	-15
4	10.59	4-Hydroxybenzoic acid	50510.04	137.1	107.9	-27
5	10.85	Aesculin	30.91	153	108.8	-18
6	11.66	Catechin	6294.43	136.9	93	-20
7	11.79	Chlorogenic acid	77.62	341.1	179.1	21
8	12.15	Cryptochlorogenic acid	30.18	288.9	109	20
9	12.22	Vanillic acid	51672.94	353.2	190.9	-20
10	12.36	Caffeic acid	145.70	353.1	172.9	-20
11	13.1	Syringic acid	303.37	166.9	107.9	-25
12	13.24	Aesculetin	1182.14	179	135	-25
13	13.44	Epicatechin	526.28	199	140.2	20
14	14.59	4-Hydroxycinnamic acid	4135.44	177	132.9	-25
15	15.79	Transpiceid	8195.52	288.8	109	-35
16	15.95	Rutin	63.34	163	119.1	-20
17	16.15	Delphinidin 3-glucoside	997.07	389.1	227.1	-25
18	18.11	Hesperidin	62.82	609.1	300.2	-46
19	18.71	Phlorizin	737.92	465.2	303.1	25
20	22.9	Naringenin	2920.60	609.2	325.3	-40

Q1: the precursor ion; Q3: the product ion.