Retinal Degeneration in a Murine Model of Retinal Ischemia by Unilateral Common Carotid Artery Occlusion

Figure 2

Abnormal retinal perfusion after UCCAO. (a) Whole retinal and optic nerve head (ONH) images showed that there was less perfusion in the retina right after UCCAO, detected with circulation of 2000 kD fluorescein isothiocyanate- (FITC-) dextran (12.5 mg/ml, 150 μL) through the left ventricle of mice for 1.5 minutes ( per group). Scale bar, 1000 and 500 (ONH) μm. (b) HIF-1α stabilization was seen in the retina a day after UCCAO ( per group). (c) A decrease in relative cerebral blood flow (CBF) was seen in the skull over the right (R) middle cerebral artery core region (dotted circles) right after UCCAO ( per group), analyzed with LSF. Ant: anterior; Mid: midline. (d) There were significant increases in hypoxia-responsive genes in the UCCAO-operated retina and RPE/choroid in comparison with those in the sham-operated retina and RPE/choroid a day after UCCAO, respectively ( per group). and . The data were analyzed using Student’s -test (two-tailed) and presented as . CH: choroid.

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