Retinal Degeneration in a Murine Model of Retinal Ischemia by Unilateral Common Carotid Artery Occlusion

Figure 6

Retinal Müller gliosis and inflammation after UCCAO. (a) Representative images and quantitative analyses showed that morphology scoring stained by GFAP with GS (referred to as Müller gliosis) in UCCAO-operated mice was significantly higher compared with that in sham-operated mice 2 weeks after UCCAO ( per group). . GCL: ganglion cell layer; INL: inner nuclear layer; ONL: outer nuclear layer. Asterisk, glial activation. White dotted squares, enlarged images. Scale bar, 100 and 40 (enlarged) μm. (b) Representative images and quantitative analyses showed that the number of IB4-positive inflammatory cells in UCCAO-operated mice significantly increased compared with that in sham-operated mice 2 weeks after UCCAO ( per group). . Asterisk, IB4-positive inflammatory cells. White dotted squares, enlarged images. Scale bar, 100 and 50 (enlarged) μm. (c) There were increasing tendencies and a significant increase in Ccl2 mRNA expression in the UCCAO-operated retina in comparison with that in the sham-operated retina for 4 weeks after UCCAO, respectively. . There was no significant change in the other genes (Ccl12 and Cox-2) on week 1 (W1), week 2 (W2), and week 4 (W4) after UCCAO ( per group). . The data were analyzed using Student’s -test (two-tailed) and presented as . IB4: isolectin GS-IB4 from Griffonia simplicifolia.

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