Inhibition of the mTORC1/NF-κB Axis Alters Amino Acid Metabolism in Human Hepatocytes

Figure 3

Rheb overexpression enhances mTORC1 signaling and NF-κB activation and increases the expression of AST, GDH, GAD, and ODC in hepatocytes. (a) The phosphorylation of mTOR and S6 was promoted by Rheb overexpression. (b) The phosphorylation of NF-κB was promoted by Rheb overexpression. (c) The relative mRNA levels of AST, GDH, GAD, and ODC were increased by Rheb overexpression. (d) The intracellular contents of AST, GDH, GAD, and ODC were increased by the overexpression of Rheb. Protein bands were quantified using Gel-Pro Analyzer 4.0 (Media Cybernetics, Inc., Rockville, MD, USA) (the values represent the , , , ).

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