Inhibition of the mTORC1/NF-κB Axis Alters Amino Acid Metabolism in Human Hepatocytes

Figure 6

Glutamate and ornithine stimulate the activation of mTORC1 and NF-κB signaling and promote the expression of AST and ODC in hepatocytes. (a) The activation of mTORC1 signaling was promoted by glutamate treatment. (b) The activation of the transcription factor NF-κB was promoted by glutamate treatment. (c) The activation of mTORC1 signaling was promoted by ornithine treatment. (d) The activation of NF-κB was promoted by ornithine treatment. (e) The relative abundance of AST mRNA was upregulated by glutamate treatment. (f) The intracellular content of AST was upregulated by glutamate. (g) The relative abundance of ODC mRNA was upregulated by ornithine treatment. (h) The intracellular content of ODC was upregulated by ornithine treatment. Protein bands were quantified using Gel-Pro Analyzer 4.0 (Media Cybernetics, Inc., Rockville, MD, USA) (the values represent the , , , ).

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