Aprepitant Promotes Caspase-Dependent Apoptotic Cell Death and G2/M Arrest through PI3K/Akt/NF-κB Axis in Cancer Stem-Like Esophageal Squamous Cell Carcinoma Spheres

Figure 4

(a) Flow cytometry analysis of cell cycle development of ESCC cell spheres treated with aprepitant. The frequency of cells in G1 (green peak), S (yellow peak), and G2/M (blue peak) was calculated with FlowJo software. (b) Aprepitant raises the percentage of the cell population in G2-M cells (c) and decreases the percentage of cell accumulation in S-phase. () indicates a statistically significant difference compared to controls, while the (^#) sign indicates a statistically significant change compared to other treatment groups including SP 500 nM and SP 1000 nM.

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