Anti-Inflammatory Effects of Antarctic Lichen Umbilicaria antarctica Methanol Extract in Lipopolysaccharide-Stimulated RAW 264.7 Macrophage Cells and Zebrafish Model

Figure 4

Effects of UA extract on the transcription and translation of interleukin- (IL-) 6 and TNF-α in LPS-induced RAW 264.7 cells. (a) Cells were treated with the indicated concentrations of UA extract for 1 h prior to incubation with LPS (0.5 μg/mL) for 24 h. The level of IL-6 and TNF-α in the supernatant was determined by enzyme-linked immunosorbent assay (ELISA). (b) Cells were treated with UA extract for 1 h and then induced with LPS for 6 h. β-Actin expression was used as an internal control for quantitative RT-PCR analysis. Three independent experiments were performed, and the data are presented as the . and compared to the cells treated with LPS alone.

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