SIRT1 Inhibits Apoptosis by Promoting Autophagic Flux in Human Nucleus Pulposus Cells in the Key Stage of Degeneration via ERK Signal Pathway

Figure 4

SIRT1 inhibits apoptosis of mild degenerative NP cells by enhancing autophagic flux. (a) LC3II/I and p62 protein levels were detected by western blot. The expression of LC3II/I and p62 was significantly increased in mildly degenerative human NP cells treated with CQ (40 μM) in combination with LV-SIRT1 transfection. (b) The apoptosis rate of mildly degenerative human NP cells evaluated by flow cytometry. The apoptosis rate was significantly increased in NP cells treated with CQ (40 μM), and there were no significant differences between NP cells treated with CQ+LV-SIRT1 and NP cells treated with CQ (40 μM). The relative protein expression of LC3II/I corresponds to the ratio of LC3II signal to LC3I signal, and p62 corresponds to the ratio of p62 signal to β-actin signal. The values are shown as the of three independent experiments performed in triplicate. , .

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