The Polymethoxy Flavonoid Sudachitin Inhibits Interleukin-1β-Induced Inflammatory Mediator Production in Human Periodontal Ligament Cells

Figure 1

Effects of sudachitin on IL-1β-induced MAPKs, NF-κB, and Akt activation in HPDLC. The cultured cells were pretreated with sudachitin (25 or 50 μM) for 1 hour and then stimulated with 1 ng/ml of IL-1β for 15, 30, or 60 min. The cell extracts were subjected to SDS-PAGE followed by Western blotting analysis with antibodies against phospho-specific p38 MAPK, p38 MAPK, phospho-specific ERK, ERK, phospho-specific JNK, JNK, phospho-specific IKK-α/β, IKK-α, phospho-specific NF-κB p65, NF-κB p65, phospho-specific Akt, Akt, and GAPDH. Each photograph is representative of the results of 3 separate experiments.