A Chimera of Th1 Stimulatory Proteins of Leishmania donovani Offers Moderate Immunotherapeutic Efficacy with a Th1-Inclined Immune Response against Visceral Leishmaniasis

Table 1

Sequences of forward and reverse primers of hamster cytokines used for real-time quantitative PCR (RT-qPCR).

Genes	Direction	Primer sequence	Product length
RPL 18	Forward	5 AACTCCACCTTCAATCAG 3	96
	Reverse	5 GATGATCCGAAAGATGAAG 3
NOS2	Forward	5 TGCCTTGCATCCTCATTGG 3	77
	Reverse	5 GTCGCTGTTGCCAGAAACTG 3
TNF-α	Forward	5 GGAGTGGCTGAGCCATCGT 3	131
	Reverse	5 AGCTGGTTGTCTTTGAGAGACATG 3
IFN-γ	Forward	5 GCTTAGATGTCGTGAATGG 3	200
	Reverse	5 GCTGCTGTTGAAGAAGTTAG 3
IL-12	Forward	5 AATTACTCTGGACGGTTCAC 3	81
	Reverse	5 GCTACTGCTGCTCTTGAC 3
TGF-β	Forward	5 ACGGAGAAGAACTGCTGTG 3	178
	Reverse	5 GGTTGTGTTGGTTGTAGAGG 3
IL-4	Forward	5 CCACGGAGAAAGACCTCATCTG 3	75
	Reverse	5 GGGTCACCTCATGTTGGAAATAAA 3
IL-10	Forward	5 GTTGCCAAACCTTATCAGAAATGA 3	102
	Reverse	5 TTCTGGCCCGTGGTTCTCT 3