Inhibition of SDF-1/CXCR4 Axis to Alleviate Abnormal Bone Formation and Angiogenesis Could Improve the Subchondral Bone Microenvironment in Osteoarthritis

Figure 3

(a–d) Immunofluorescent staining and quantitative analysis of osterix+/nestin+ cells in the subchondral bone. Scale bar, 50 μm. (e, f) Immunofluorescent staining shows p-ERK activity in the subchondral bone, inhibition of SDF-1 signaling downregulated p-ERK expression in vivo. Scale bar, 50 μm. (g, h) Blocking the SDF-1 signaling reduces the number of TRAP+ osteoclasts in the subchondral bone. Scale bar, 100 μm. (i, j) Immunohistochemical staining and quantitative analysis of RUNX2 in different groups. Scale bar, 100 μm. , .

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