Inhibition of SDF-1/CXCR4 Axis to Alleviate Abnormal Bone Formation and Angiogenesis Could Improve the Subchondral Bone Microenvironment in Osteoarthritis

Figure 4

(a) The absorbance of CCK8 after treatment with SDF-1 (20 ng/ml, 50 ng/ml) or AMD3100 (200 ng/ml). (b) After treatment with SDF-1 (20 ng/ml, 50 ng/ml) or AMD3100 (200 ng/ml), the cell viability calculated by formula, , (dosing): absorbance of the well with cells, CCK8 solution and drug; A (blank): absorbance of the pore with medium and CCK8 solution, but no cells; A (0 dosing): absorbance of the well with cells, CCK8 solution, but no drug. (c, d) The proliferation effect of SDF-1 on HUVECs detected by EdU and quantitative analysis. Scale bar, 100 μm. (e, f) The microscopic observation of Transwell assay shows that SDF-1 significantly induces HUVEC migration. , , ., .

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