Inhibition of SDF-1/CXCR4 Axis to Alleviate Abnormal Bone Formation and Angiogenesis Could Improve the Subchondral Bone Microenvironment in Osteoarthritis

Figure 5

(a, b) Double immunofluorescence staining shows inhibition of SDF-1 signaling restored CD31⁺Emcn⁺ blood vessels similar to sham controls. Scale bar, 100 μm/50 μm. (c, d) HUVECs treated with SDF-1(20 ng/ml, 50 ng/ml) or AMD3100 (200 ng/ml) in tube formation experiment. Scale bar, 50 μm. (e) The result of western blot of HUVECs treated with SDF-1(20 ng/ml, 50 ng/ml) for 30 min with or without 2 hr pretreatment of AMD3100 (200 ng/ml). , , . , , .

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