Research Article
Human Dental Pulp-Derived Mesenchymal Stem Cell Potential to Differentiate into Smooth Muscle-Like Cells In Vitro
Table 1
List of primers used to evaluate the marker (genes) expression profiling of DPSCs using RT-PCR.
| | Gene | Primer sequence | Product size (bp) | Accession no. |
| | OCT4 | F: AAGCAGCGACTATGCACAAC
R: AGTACAGTGCAGTGAAGTGAGG | 140 | NM_002701.5 | | SOX2 | F: CACCCACAGCAAATGACAGC
R: AGTCCCCCAAAAAGAAGTCCAG | 120 | NM_003106.3 | | NANOG | F: GCAGATGCAAGAACTCTCCAAC
R: CTGCGTCACACCATTGCTATTC | 133 | AB093576.1 | | CEBP-α | F: GAAGTCGGTGGACAAGAAC
R: CATTGTCACTGGTCAGCTC | 140 | NM_004364.4 | | PPAR-γ | F: TTGCTGTCATTATTCTCAGT
R: GAGGACTCAGGGTGGTTCAG | 124 | AB565476.1 | | FABP4 | F: TGAGATTTCCTTCATACTGG
R: TGGTTGATTTTCCATCCCAT | 128 | NM_001442.2 | | ON | F: GTGCAGAGGAAACCGAAGAG
R: AAGTGGCAGGAAGAGTCGAA | 202 | J03040.1 | | BMP2 | F: TAGACCTGTATCGCAGGCAC
R: GGTTGTTTTCCCACTCGTTT | 149 | NM_001200.2 | | RUNX-2 | F: ATGTGTGTTTGTTTCAGCAG
R: TCCCTAAAGTCACTCGGTAT | 199 | NM_001024630.3 | | α-SMA | F: ACTGGGACGACATGGAAAAG
R: CATACATGGCTGGGACATTG | 168 | BC093052.1 | | SM22-α | F: AGCCTTCTTTCCCCAGACAT
R: CACCAGCTTGCTCAGAATCA | 216 | D17409.1 | | CALP | F: TGACCCCAAGAACCATATCC
R: CAGGGACATGGAGGAGTTGT | 169 | BC141833.1 | | SMTN | F: CTGGGCAGTGTCACTCATGT
R: CTGATCCAGCATCTTGTCCA | 234 | NM_001207018.2 | | MHY-11 | F: CAGCCAGCATTAAGGAGGAG
R: AAGTACCGCTCCCTCAGGTT | 190 | BC031040.1 | | YWHAZ | F: ACGAAGCTGAAGCAGGAGAAG
R: TTTGTGGGACAGCATGGATG | 111 | BC108281.1 |
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