Acetyl-L-Carnitine Induces Autophagy to Promote Mouse Spermatogonia Cell Recovery after Heat Stress Damage

Figure 3

Effect of ALC on heat stress-induced autophagy in GC-1 cells. (a) Detection of autophagosomes using monodansylcadaverine (MDC) staining. (b) Percentages of MDC-positive cells. (c) mRNA levels of Atg5, Beclin1, and LC3II in GC-1 cells. (d, e) Western blot analysis of LC3II/LC3I protein expression in GC-1 cells. (f, g) Western blot analysis of Beclin1 protein expression in GC-1 cells. (h) The immunofluorescence of LC3II in GC-1 cells. (i) The relative AOD of the LC3II positive cells. (j, k) Western blot analysis of p62 protein expression in GC-1 cells. (l) The mRNA levels of lysosomal biogenesis genes were determined using RT-PCR. The data are presented as the , , , . CON: control group; HS: recover at 6 h; HS-ALC: recover at 6 h with ALCAR.

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