Dilation of Brain Veins and Perivascular Infiltration by Glioblastoma Cells in an In Vivo Assay of Early Tumor Angiogenesis

Figure 2

(a) Microphotographs of the CW performed before and 1 to 2 weeks after grafting of U87MG cells. Over this time frame, hypertrophy of the venous-venous anastomoses can be assessed without intervening hemorrhages that may obscure the phenomenon. (b) Graph showing the time course of diameter of arterial and venous vascular structures after grafting. One week after grafting, the diameter of venous-venous anastomoses and cortical veins increased significantly compared to their pregrafting diameter ( and , respectively; paired Student’s test). Two weeks after grafting, the diameter of venous-venous anastomoses and cortical veins increased significantly compared to their pregrafting diameter ( and , respectively; paired Student’s test). Three weeks after grafting, the vasculature was chaotic and heterogeneous with large-caliber vessels, arterialized veins, and hemorrhages.

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