Dilation of Brain Veins and Perivascular Infiltration by Glioblastoma Cells in an In Vivo Assay of Early Tumor Angiogenesis

Figure 5

Immunofluorescence microscopy showing the expression of the BBB marker Glut1 and IgG extravasation in GFP-expressing U87MG brain xenografts. (a) At 2 weeks after grafting, the expression of Glut1 (red) by peritumor vessels is still present (white arrow). Scale bar, 70 μm. (b) At 3 weeks after grafting, Glut1 expression (white) by vessels within and around the tumor is heavily disrupted (yellow arrow) with extravasation of IgG (red, yellow star). Scale bar, 100 μm.

(a)

(b)