Inhibitory Effect of Multimodal Nanoassemblies against Glycative and Oxidative Stress in Cancer and Glycation Animal Models

Table 1

Stepwise method for the inhibitory effect of nanoassemblies in the glycation animal model.

Step	Experiment (dosing) (30 days)	Group 1 ()	Group 2 ()	Group 3 ()	Group 4 ()
Step 1 (acclimatization for one week)	Diet and normal water	Pellet diet/water	Pellet diet/water	Pellet diet/water	Pellet diet/water
Step 2 (after overnight fasting)	FBG	Check FBG after overnight fasting	Check FBG after overnight fasting	Check FBG after overnight fasting	Check FBG after overnight fasting
Step 3	Alloxan induction/booster dose after one week (if required)	No induction	100 mg/kg alloxan induction	100 mg/kg alloxan induction	100 mg/kg alloxan induction
Step 4	Nanoassembly treatment after one dosing of alloxan	No treatment	No treatment	5 μM of PFT-HSA-TFT-Cy7	5 μM of PFT-Hcy-HSA-Cy7
Step 5	Glucose	Normal water	10% glucose in drinking water daily for 30 days	10%glucose in drinking water daily for 30 days	10% glucose in drinking water daily for 30 days

FBG: fasting blood glucose.