Inhibitory Effect of Multimodal Nanoassemblies against Glycative and Oxidative Stress in Cancer and Glycation Animal Models

Table 3

Protocol of cDNA synthesis.

Reagents and samples	Volume
Control GAPDH RNA (50 ng/μl)	2 μl
Oligo (dT)18 primer or random hexamer primer or reverse GAPDH primer	1 μl
5× reaction buffer	4 μl
RiboLock RNase inhibitor (20 U/μL)	1 μl
10 mM dNTP mix	2 μl
RevertAid RT (200 U/μL)	1 μl
Water, nuclease-free	9 μl
Total volume	20 μl