Knockdown of MALAT1 Inhibits the Progression of Chronic Periodontitis via Targeting miR-769-5p/HIF3A Axis

Figure 1

Inhibition of metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) attenuated lipopolysaccharide- (LPS-) induced periodontal ligament cell (PDLC) injury. (a) The expression of MALAT1 was detected by quantitative real-time polymerase chain reaction (qRT-PCR) in gingival tissues of patients with chronic periodontitis (CP) and healthy controls. vs. healthy controls. (b) The expression of MALAT1 was detected by qRT-PCR in LPS-treated PDLCs and control cells. vs. control cells. (c) The expression of MALAT1 was detected by qRT-PCR in PDLCs transfected with si-NC or si-MALAT1. vs. si-NC. (d) Cell viability was detected by MTT assay in LPS-treated PDLCs transfected with si-NC or si-MALAT1. vs. control; ^## vs. si-NC. (e–g) The concentrations of interleukin- (IL-) 6, IL-1β, and tumor necrosis factor-α (TNF-α) were measured by enzyme-linked immunosorbent assay (ELISA) in supernatants of LPS-treated PDLCs transfected with si-NC or si-MALAT1. vs. control; ^## vs. si-NC. (h) The protein levels of Bax and Bcl-2 were detected by western blot in LPS-treated PDLCs transfected with si-NC or si-MALAT1. vs. control; ^## vs. si-NC. (i) The protein level of caspase-3 was detected by western blot in LPS-treated PDLCs transfected with si-NC or si-MALAT1. vs. control; ^## vs. si-NC.

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