Knockdown of MALAT1 Inhibits the Progression of Chronic Periodontitis via Targeting miR-769-5p/HIF3A Axis

Figure 5

MALAT1 knockdown inhibited LPS-induced PDLC injury through regulating the miR-769-5p/HIF3A axis. (a) The expression of HIF3A was detected by qRT-PCR in gingival tissues of patients with CP and healthy controls. vs. healthy controls. (b) The expression of HIF3A was detected by western blot in LPS-treated PDLCs and control cells. vs. control cells. (c) The expression of HIF3A was detected by western blot in LPS-treated PDLCs transfected with si-NC, si-MALAT1, or si-MALAT1+miR-769-5p inhibitor. vs. si-NC; ^## vs. si-MALAT1. (d) Cell viability was detected by MTT assay in LPS-treated PDLCs transfected with si-NC, si-MALAT1, miR-769-5p inhibitor, or pcDNA-HIF3A. vs. si-NC; ^## vs. si-MALAT1. (e–g) The concentrations of IL-6, IL-1β, and TNF-α were measured by ELISA in supernatants of LPS-treated PDLCs transfected with si-NC, si-MALAT1, miR-769-5p inhibitor, or pcDNA-HIF3A. vs. si-NC; ^## vs. si-MALAT1. (h) The protein levels of Bax and Bcl-2 were detected by western blot in LPS-treated PDLCs transfected with si-NC, si-MALAT1, miR-769-5p inhibitor, or pcDNA-HIF3A. vs. si-NC; ^## vs. si-MALAT1. (i) The protein level of caspase-3 was detected by western blot in LPS-treated PDLCs transfected with si-NC, si-MALAT1, miR-769-5p inhibitor, or pcDNA-HIF3A. vs. si-NC; ^# and ^## vs. si-MALAT1.

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