miR-193a-3p Promotes the Invasion, Migration, and Mesenchymal Transition in Glioma through Regulating BTRC

Figure 4

Effects of miR-193a-3p on the MT, migration, and invasion of glioma cells. (a) Expression level of miR-193a-3p in GSE26576. (b) Correlation curve between the expression level of miR-193a-3p and BTRC in glioma ( and ). (c) Transfection efficiency of miR-193a-3p in LN-229 evaluated by qRT-PCR. (d) Expression of BTRC in normal glial cells and high-grade glioma tissues in HPA. (e) Invasion and migration abilities of LN-229 cells transfected with miR-NC and miR-193a-3p mimic groups analyzed by transwell assay. Scale bars, 100 μm. (f) Western blot analyses for T-cadherin, N-cadherin, and BTRC in LN-229 cells transfected with miR-NC and miR-193a-3p mimics. (g) Migration capability of LN-229 cells transfected with NC and miR-193a-3p mimics analyzed by wound-healing assay at 0 and 24 h. Scale bars, 500 μm. Data are shown as the based on three independent experiments. and .

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