miR-193a-3p Promotes the Invasion, Migration, and Mesenchymal Transition in Glioma through Regulating BTRC

Figure 5

Effects of ectopic expression of miR-193a-3p and BTRC on the cell invasion, migration, and MT of glioma. (a) BTRC with WT 3UTR or MUT 3UTR was constructed into a luciferase reporter vector and cotransfected with miR-193a-3p mimics in LN-229 cells. Relative luciferase activity determined 48h after transfection. (b) LN-229 cells transfected with miR-NC, miR-193a-3p inhibitor, si-NC, and si-BTRC plasmid. The transwell assay was used to analyze the migration and invasion abilities of LN-229 cells in NC-inhibitor+si-NC, miR-193a-3p-inhibitor+si-NC, NC-inhibitor+si-BTRC, and miR-193a-3p-inhibitor+si-BTRC groups, and the cell number was statistically analyzed. Scale bars, 100μm. (c) Migration ability in NC-inhibitor+si-NC, miR-193a-3p-inhibitor+si-NC, NC-inhibitor+si-BTRC, and miR-193a-3p-inhibitor+si-BTRC groups detected by wound-healing assay, and the migration distance was statistically analyzed. Scale bars, 500μm. (d) Expression levels of BTRC, T-cadherin, and N-cadherin detected by Western blot. Data are shown as the based on three independent experiments. and .

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