Research Article
Relationship of PD-1 (PDCD1) and PD-L1 (CD274) Single Nucleotide Polymorphisms with Polycystic Ovary Syndrome
Table 1
SNP sites and primer sequences of PD-1 gene and PD-L1 gene.
| | SNP sites | Polymorphism | Primers | Product length | Extension | Products | Extension primer |
| | rs10204525 | (A/G) | F: CAGGGAAGCTGAGGCAGTAA
R: TTTCAGGAATGGGTTCCAAG | 272 bp | Forward | (A/G) | TTTTTTACCTAGGGCCCCCCAT | | rs4742095 | (G/T) | F: CCCACAGCCCTTCTTGTGAA
R: TCCAGAGTAGGATACTATAGCCAGT | 277 bp | Reverse | (A/C) | GGCAGTATGTTGACAGTATTTCAAAG | | rs702275 | (G/T) | F: TCTTCCTTGATTTCCTTTTATCCA
R: GCAATGAAAAGCCACCAGAT | 236 bp | Reverse | (A/C) | TTTTTTGAAAATCTTTAGGAGCTGTTTCTGTAG | | rs822339 | (C/T) | F: TAACTCTGGCCCAAGGAAAA
R: TTTTGGTCTGTTTATGTCACTGG | 250 bp | Reverse | (A/G) | TTTTTTTTTTTTTTTCATCTACAGGATAGACGGAAAGGA | | rs2282055 | (A/C) | F: TGTAGGGGGAAAAAGCATTG
R: GCCCACAGCCACATAAACTT | 258 bp | Reverse | (G/T) | TTTTTTTTTTTTTTTTTTTTTTGTCAGATTCTCCTTGCTCTGAAAT | | rs6415794 | (A/T) | F: CCATCAATCTGAGGGCTAACA
R: GATGTCATGAATGGAGAAGCA | 264 bp | Forward | (A/T) | TTATGTTGTATTTCTGGTCCCTGAT | | rs2890658 | (A/C) | F: CCTGGGCAACAGAGAAAGAC
R: GGAATGGCGAGATACCTGTG | 306 bp | Forward | (A/C) | TTTTTTAAGAGGAAGTGAAATAATCAAGGC |
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