Long Noncoding RNA OIP5-AS1 Promotes the Disease Progression in Nasopharyngeal Carcinoma by Targeting miR-203

Figure 5

OIP5-AS1 directly binds to miR-203 and suppresses miR-203 expression. (a) The relative expression of miR-342-3p, miR-422a, miR-204-5p, and miR-203 in 5-8F and CNE-1 cells after knockdown of OIP5-AS1. (b) The relative expression of miR-203 in NPC tissues and adjacent normal tissues obtained from 105 patients. (c) The relative expression of miR-203 in NPC line cells and the nasopharyngeal epithelium cell NP69. (d) Pearson correlation analysis revealing a significantly negative relationship between miR-203 and OIP5-AS1 in NPC patient specimens (, ). (e, f) Western blot analysis of the expression of ZEB2, E2F3, and CDH6 in 5-8F and CNE-1 cells with or without knockdown of OIP5-AS1. Relative expression of ZEB2, E2F3, and CDH6 was normalized to GAPDH. (g) starBase 3.0 analysis revealing the specific binding site of miR-203 in OIP5-AS1. Relative luciferase activity in 293T cells after cotransfected with OIP5-AS1 (WT or MUT) and miR-203 mimic (or miR-NC). WT: wild type; MUT: mutant type; NC: negative control. , .

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