PICK1 Deficiency Exacerbates Sepsis-Associated Acute Kidney Injury

Figure 5

Inhibition of PICK1 expression could increase LPS-induced apoptosis, and NAC could alleviate pretreatment-induced apoptosis. (a, b) After 24 hours of LPS treatment in the different groups, apoptosis rate was detected by flow cytometry (WT versus WT+LPS, NC+LPS versus sh-PICK1+LPS; ). (c–e) HK2 cells were pretreated with 10 mM NAC for 1 h and then treated with LPS for another 24 h. Representative images of ROS and apoptosis rate in flow cytometry after NAC and LPS treatment (sh-PICK1+LPS versus sh-PICK1+LPS+NAC, ). (f) After treatment with NAC, the ASK1 and p38MAPK were detected by western blotting (sh-PICK1+LPS versus sh-PICK1+LPS+NAC; , ). (f) After treatment with NAC, the apoptotic protein was detected by western blotting (sh-PICK1+LPS versus sh-PICK1+LPS+NAC, ).

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