LDHA-Mediated Glycolytic Metabolism in Nucleus Pulposus Cells Is a Potential Therapeutic Target for Intervertebral Disc Degeneration

Figure 3

Decrease of SIRT1 expression will aggravate the progress of intervertebral disc degeneration. (a) The transfection efficiency of nucleus pulposus cells was over 80%，,  μg/ml. (b) Flow cytometry assay showed that reducing the expression level of SITRT1 resulted in a decrease in the proliferation rate and an increase in the apoptosis rate. . (c) EdU cell proliferation assay (200x). (d) Reducing the expression level of SIRT1 leads to accelerated senescence of NP cells (SA-β-gal staining (200x)).(e) The SIRT1, Bax, Bcl-2, cleaved caspase 3, and senescence-related protein (P53, P21, and P16) levels were analyzed by western blotting. versus the control group.  μm.

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