[Retracted] Synthesis, Characterization, and Specific Localization of Mitochondrial-Targeted Antioxidant Peptide SS31 Probe

Figure 3

Cellular uptake of FITC-SS31. (a) Flow cytometry data indicated that the mean fluorescence intensity of FITC-SS31 in the 4 h group () was higher than that in the 2 h group () in normal cells, and the results were fold change to 0 h. In the oxidative damaged cells, the mean fluorescence intensity in the 2 h group () was higher than that in the 4 h group (), and the results were fold change to 0 h. (b) The mean fluorescence intensity of the normal 661W cells and oxidative damaged cells after incubating with FITC-SS31 fluorescent probes for 2 h and 4 h detected by flow cytometry, respectively (, ; significantly different from control; ^#significantly different from the 2 h group). (c) As demonstrated by flow cytometry analysis, the positive rate of the concentration of the 10 μM group was greater than that of the 1 μM group and 5 μM group, both in normal and oxidative cells. (d) The positive rate of the FITC-SS31 probe incubated with the 661w cells at different concentrations (, ; significantly different from control; ^#significantly different from the 1 μM group; ^##significantly different from the 5 μM group).

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