[Retracted] Synthesis, Characterization, and Specific Localization of Mitochondrial-Targeted Antioxidant Peptide SS31 Probe

Figure 4

Confocal microscopy showing subcellular localization of FITC-SS31 in 661W cells. Treatment with a fluorescent probe at 10 μM (a) or 100 nM (c) for 2 h and MitoTracker Red (1 : 3000) for 30 min at 37°C, green stain: FITC-SS31, red stain: MitoTracker Red. FITC-SS31 is colocalized to the mitochondria (scale bars: 25 μm). The oxidative groups were treated with 100 μM H₂O₂. (b) Quantitative colocalization image analyses using the overlap coefficient in IPP illustrated colocalization between FITC-SS31 and MitoTracker Red, and the value of the overlap coefficient was determined for 10 μM FITC-SS31 at [() %] for control groups and [() %] for oxidative groups (). (d) The value of the overlap coefficient for 100 nM FITC-SS31 at [() %] for control groups and [() %] for oxidative groups ().

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